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KMID : 0364820160520040444
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Korean Journal of Microbiology
2016 Volume.52 No. 4 p.444 ~ p.454
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Isolation and characterization of lactic acid bacteria for use as silage additivesSixteen lactic acid bacterial strains were isolated from silage and cow dung samples, and characterized to identify their
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Ro Yu-Mi
Lee Gwan-Hyeong
Park In-Cheol
Kim Wan-Gyu
Han Byeong-Hak
Yoo Jae-Hong
Ahn Jae-Hyung
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Abstract
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Sixteen lactic acid bacterial strains were isolated from silage and cow dung samples, and characterized to identify their potential as silage additives. They were identified as the members of the genera Lactobacillus, Enterococcus, and Weissella, and clustered into nine groups based on the sequences of the genes for 16S rRNA, RNA polymerase alpha subunit, 60-kDa heat shock protein, and phenylalanyl-tRNA synthase alpha subunit. Among them, the three strains which were genetically similar to L. plantarum showed the fastest growth and pH decrease in MRS and rye extract media, the highest numbers of available carbohydrates, and the widest ranges of pH, temperature, and salinity for growth. In addition, they showed no amplified DNA products in the PCR examination targeting the genes for the production of biogenic amines, and the MRS media where they had been cultured showed relatively high inhibition effect against the growth of silage-spoiling microorganisms, including fungi, yeast, and clostridia. The results suggest that these strains are good candidates for silage additives. However, the rye extract media where the lactic acid bacteria had been cultured had no effect on or stimulated the growth of the silage-spoiling microorganisms, and the causes must be established for the practical use of the lactic acid bacteria as silage additives.
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KEYWORD
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antimicrobial activity, lactic acid bacteria, silage
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